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SignalP and TMHMM

The SignalP and TMHMM plugin contains tools for finding secretory signal peptides and predicting transmembrane helices in protein sequences.

This plugin contains two tools: Signal Peptide Prediction and Transmembrane Helix Prediction. When installed, these tools are placed in the Toolbox under the Protein Analyses folder.

An active internet connection is required to run these tools.

Signal Peptide Prediction

Signal peptides target proteins to the extracellular environment either through direct plasmamembrane translocation in prokaryotes or are routed through the endoplasmatic reticulum in eukaryotic cells. The signal peptide is removed from the resulting mature protein during translocation across the membrane.

For the prediction of signal peptides, we query SignalP-NN [Nielsen et al., 1997] [Bendtsen et al., 2004a] [Teufel et al. 2022] located at https://services.healthtech.dtu.dk/services/SignalP-6.0/.

The predictions obtained can be shown as annotations on the sequence or in a table.

Transmembrane Helix Prediction

Similarly, the Transmembrane Helix Prediction tool will annotate the input protein sequence with show predicted transmembrane helices and in addition to inside and outside regions. Detailed text output from DeepTMHMM can also be generated.

This tool uses DeepTMHMM, located at: https://dtu.biolib.com/DeepTMHMM.

Additional information about SignalP and TMHMM can be found at https://services.healthtech.dtu.dk.

 

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23.0.0

QIAGEN CLC Genomics Workbench


 [23.9.9, 23.0.3, 23.0.1, 23.0]

QIAGEN CLC Main Workbench


 [23.0.1, 23.0]

22.0.0

QIAGEN CLC Genomics Workbench


 [22.0.3, 22.0.2, 22.0.1, 22.0]

QIAGEN CLC Main Workbench


 [22.0.3, 22.0.2, 22.0.1, 22.0]

21.0.1

QIAGEN CLC Genomics Workbench


 [21.0.6, 21.0.5, 21.0.4, 21.0.3, 21.0.2, 21.0.1, 21.0]

QIAGEN CLC Main Workbench


 [21.0.6, 21.0.5, 21.0.4, 21.0.3, 21.0.2, 21.0.1, 21.0]

20.0.0

QIAGEN CLC Genomics Workbench


 [20.0.5, 20.0.4, 20.0.3, 20.0.2, 20.0.1, 20.0]

QIAGEN CLC Main Workbench


 [20.0.5, 20.0.4, 20.0.3, 20.0.2, 20.0.1, 20.0]

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