Mean reference gene coverage value in QC for RNAScan Panels report is too high

Issue description

The value for the “Mean read coverage per reference gene control primer” in QC for RNAscan Panels reports generated using the Biomedical Genomics Analysis 20.2 plugin is 100 times too high. This has the knock-on effect that the “Target gene vs reference gene coverage (%)” value in these reports is 100 times too low.

Recommendations

For reports generated using Biomedical Genomics Analysis 20.2,

• Divide the value of “Mean read coverage per reference gene control primer” by 100 to get the correct value
• Multiply the value of “Target gene vs reference gene coverage (%)” by 100 to get the correct value.

These adjustments should not be applied to reports generated by earlier versions of the Biomedical Genomics Analysis plugin, as those are not affected by this issue.

Affected software

Biomedical Genomics Analysis 20.2. The following ready-to-use workflows include QC for RNAscan Panels:

• Detect QIAseq RNAscan Fusions
• Perform QIAseq Multimodal Analysis (Illumina)
• Perform QIAseq Multimodal Analysis with TMB and MSI (Illumina)
• Perform QIAseq RNA Fusion XP Analysis

Earlier versions of this plugin are not affected, and the issue has been addressed in Biomedical Genomics Analysis 21.0.