Complete NGS workflows for the Element AVITI System Benchtop Sequencer

QIAseq Panels set a new standard for NGS genotyping. Major innovations within this chemistry provide improved ease-of-use, efficiency and expanded variant analysis to simplify and speed-up your NGS workflows.

• Complete, uniform target coverage – single-tube enrichment for up to 20,000 DNA targets (and an
  additional 8,000 RNA targets for multimodal) staggered placement of primers across the target region
  ensures high uniformity (>99%) and complete coverage.
• High compatibility with challenging samples – works with FFPE samples, plasma/serum, fresh or frozen
  tissue, cell lines, liquid biopsies with a minimum DNA input requirement as low as 10 ng.

• Single-day, automation-friendly library preparation – go from sample to sequencing-ready library in 8 hours or less with minimum hands-on time.
• Customization made easy – boost any cataloged panel with additional content if something is missing or design a custom panel using either our flexible GeneGlobe custom builder or expert design service.
• Pan-cancer profiling with multimodal approach – comprehensive genomic profiling workflow for simultaneous detection ofDNA variants, RNA fusions and TMB/MSI status in solid tumors and heme malignancies.

The AVITI™ System from Element Biosciences is an out-of-the-box, plug-and-run benchtop sequencer offering an unrivaled combination of performance, cost, and flexibility. Element’s proprietary Avidity Sequencing™ chemistries enable exceptional accuracy and cost efficiency compared to other benchtop systems. With two random access flow cells, the AVITI™ System is equivalent to having two sequencing systems in one.

• Industry leading accuracy - With > 90% Q30 at 2x150 read length, the AVITI™ System delivers exceptional sequencing accuracy for a comprehensive range of NGS applications.
• Simple to use - The AVITI™ empowers labs of all sizes and NGS experience with guided workflows, comprehensive run parameters and flexible system settings.
• Disruptive pricing - Avidity Sequencing technology lowers operating costs with an average of $2-5 per Gb and $0.6-1 per million reads.

LightSpeed is a new module for QIAGEN CLC Genomics Workbench Premium that empowers laboratories to perform NGS secondary analysis with high accuracy at unprecedented runtimes. FASTQ files produced from the AVITI™ System are uploaded to LightSpeed. Within minutes (or less), LightSpeed produces VCF files containing single nucleotide variants (SNV), insertion–deletion mutation (InDel) and structural variant (SV) calls. The module is deployable using local computers or Amazon Web Services (AWS®) cloud and performs quality and adapter trimming, read mapping, deduplication, local realignment, quality control and variant calling.

• Agnostic – Analysis workflows are custom-tailored to panels from any vendor.
• Ultra-rapid - Requires less than 20 minutes to process one WGS sample at 35x coverage.
• Cost-efficient - Significantly reduces computing costs, requiring less than $0.40 per WGS sample.

QCI Interpret is an out-of-the-box, fully customizable software solution that enables NGS variant interpretation and reporting for oncology and hereditary applications. VCF files from QCI Secondary Analysis are directly uploaded to QCI Interpret. Users then select a preconfigured workflow, and QCI Interpret automatically filters variants and computes classifications according to ACMG and AMP/ASCO/CAP professional guidelines.

Unlike other tertiary analysis solutions, QCI Interpret uses augmented molecular intelligence (the combination of human and machine curation) to ensure the evidence being considered is accurate, consistent and up-to-date. Users can then review the literature and criteria supporting each variant classification, determine reportability status, and confidently generate a final, patient-specific report with trusted diagnostic, prognostic and therapeutic information.

View a sample report here.